Influenza-binding sialylated polymer coated gold nanoparticles prepared via RAFT polymerization and reductive amination

Zhiyue Zhang, Bert Schepens, Lutz Nuhn, Xavier Saelens, Michael Schotsaert, Nico Callewaert, Riet De Rycke, Q. Zhang, Sébastien Moins, Samira Benali, Laetitia Mespouille, Richard. Hoogenboom, and Bruno. G. De Geest


We report on a straightforward strategy to fabricate bioactive glycosylated gold nanoparticles via a combination of RAFT polymerization, carbohydrate ligation through reductive amination and thiol–gold self-assembly. This approach is used for the design of gold nanoparticles decorated with the complex sialylated glycan Neu5Ac-α-2-6-Gal, and we demonstrate multivalent and specific recognition between the nanoparticles, lectins and hemagglutinin on the surface of the influenza virus.